Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQCYTDVTEFAIPVSTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLEQAFTEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSLNQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG 2X6N Chain:A ((13-160)) FDKFFMDYIGPLPPS-QGYLYVLVVVDGMTGFTWLYPTKA-PSTSATVKSL-NVLTSI-AIPRVIHSDQGAAFTSSTFAEWAKERGIHLEFSTPYHPQSGSKVERKNSDMKRLLTKL---LVGRPTKWYDLLPVVQLAMNN-TYSPVLK-YTPHQLL-----

General information: TITO was launched using: RESULT: Template: 2X6N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 643 -58354 -90.75 -394.28 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -90.75 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.352

(partial model without unconserved sides chains): PDB file : Tito_2X6N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X6N-query.scw PDB file : Tito_Scwrl_2X6N.pdb: