Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESKISILDRLYSWIYIKKLKKLGAIIEENVVICFGAKLFFNEECLIQKDTVIGRFVLIEANRITIGNNCLFFPRTLIYSKETFSLGTRGKISKDCIFRANKINIGREFWCNEAVRIGEGGWNQKSANIKIGDYQFIGPRAQINVSDSVELMGYGGLGIETMI--FTHGAGHGQSATDGFYA--EQNKVIIQKNVSILTRAIILPGVIVSQGTTVAANAIVTKSFPKHSLIGGVPARYIGQSNKEISVKEQKNIIVDILKEGLGTEPVIKNNSFCFEKFNENITFQYDLEKIESTDNISQRDIIIFYQGTNKCHKNYSTCIDLKSKTISGRASKASEFLRDKFRRKGIILNYKNYSPFSLNYDYLIINKIEV 3FSC Chain:A ((12-221)) ---------------------KEGVIIGENVTI--EDNVYIDYGCIIRDNVHIKKGSFIGA-RSILGEYLVDFYNDRINKKHPLIIGENALIRTENVIYGDTI-IGDNFQTGHKVTIREN--TKIGNNVKIGTLSDI--QHHVYIGNYVNIHSNVFVGEKSIIKDFVWLFPHVVLTNDPTPPSNELLGVTIELFAVIAARSVVLPGIHINEDALVGAGAVVTKDVPKETVVVGNPAREI-------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3FSC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1293 -103629 -80.15 -503.05 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -80.15 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.380

(partial model without unconserved sides chains): PDB file : Tito_3FSC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FSC-query.scw PDB file : Tito_Scwrl_3FSC.pdb: