Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MRSGRDKKIAGVCAGVAHYLDM---------DPTIVRVIWGVLTC-----------CYGAGIVAYIILWIIAPVATDY--- 2M4E Chain:A ((1-86)) MSKTAKLNNEEKLVKKALEIGGKMAKMQGFDLPQSPQPVRVKAVYLFLVDAKQIAPLPDSKLDGANIKHRLALWIHAALPDNDPLK

General information: TITO was launched using: RESULT: Template: 2M4E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 107 -6762 -63.20 -116.59 target 2D structure prediction score : 0.83 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -63.20 2D Compatibility (Sec. Struct. Predict.) : 0.83 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_2M4E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2M4E-query.scw PDB file : Tito_Scwrl_2M4E.pdb: