Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSFIFNGIGRSNCVCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKAQKKEKIKVNKSNFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEPKKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAKQYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELEKDQRIASQDTIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDHLIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI 4H62 Chain:V ((13-27)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------QIEELLDNCIETFVA----

General information: TITO was launched using: RESULT: Template: 4H62.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain V - contact count / total energy / energy per contact / energy per residue : 2 -837 -418.50 -55.80 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain V : 0.58 3D Compatibility (PKB) : -418.50 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.684

(partial model without unconserved sides chains): PDB file : Tito_4H62.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H62-query.scw PDB file : Tito_Scwrl_4H62.pdb: