Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEYLAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKIVFEPVSELLK 2IAZ Chain:B ((1-112)) MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTDYLAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKIVFEPVSELLK

General information: TITO was launched using: RESULT: Template: 2IAZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 199 -24319 -122.21 -217.13 target 2D structure prediction score : 0.87 Monomeric hydrophicity matching model chain B : 1.00 3D Compatibility (PKB) : -122.21 2D Compatibility (Sec. Struct. Predict.) : 0.87 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.664

(partial model without unconserved sides chains): PDB file : Tito_2IAZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IAZ-query.scw PDB file : Tito_Scwrl_2IAZ.pdb: