Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSGLILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGTMDMVVERNAYGRQLGSFYTEAECKGVGK-IPMTFIRGPIISSVGEGVEILAIVNNQIVAAQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS 4WXY Chain:B ((1-188)) MKIGVLGLQGAVREHVRAIEACGAEAVIVKKSEQLE----GLDGLVLPGGESTTMRRLIDRYGLMEPLKQFAAAGKPMFGT-AGLILLAKRIVGYDEPHLGLMDITVERNSFGRQRESFEAELSIKGV-DGFVGVFIRAPHIVEAGDGVDVLATYNDRIVAARQGQFLGCSFNPELTDDHRLMQYFLNMVKE--

General information: TITO was launched using: RESULT: Template: 4WXY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1081 -111055 -102.73 -600.29 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : -102.73 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.712

(partial model without unconserved sides chains): PDB file : Tito_4WXY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WXY-query.scw PDB file : Tito_Scwrl_4WXY.pdb: