Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGLAANMIGVQKRVIIFNLG---------LVP-VVMFNPVLLSF-EGSYEAEEGCLSLVGVR-STKRYETIRLAYRDSKWQEQTITLTGFPAQICQHELDHLEGRII 5KOB Chain:C ((39-158)) -------------------------LVKDMFETM--HDANGAGLAAPQIGVNLQVVIFGFGHNERYPDAPPVPETVLINPTITPVSQDMEEGWEGCLSVPGLRGAVSRFSMIKYHGFDQYGKPIDRVAEGFHARVVQHECDHLIGKL-

General information: TITO was launched using: RESULT: Template: 5KOB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 452 -42238 -93.45 -391.09 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.71 3D Compatibility (PKB) : -93.45 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_5KOB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KOB-query.scw PDB file : Tito_Scwrl_5KOB.pdb: