Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELV-PQEAIRQSAVFLLFVGDLNRAEKGARLHT-DTF-QPQGVEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMALGVPNQTHDVKPRLPLDNVVFEEEYQEQS--TEAIQAYDRVQADYAGARAT----TSWSQRLAEQFGQAEPSSTRKNLEQKKLL 1ZCH Chain:A ((1-245)) MNEVIKSLTDHRSIRSYTDEPVAQEQLDQIIEAVQSAPSSINGQQVTVITVQDKERKKKISELAGGQPWIDQAPVFLLFCADFNRAKIALEDLHDFKMEITNGLESVLVGAVDAGIALGTATAAAESLGLGTVPIGAVRGNPQELIELLELPKYVFPLSGLVIGHPADRSAKKPRLPQEAVNHQETYLNQDELTSHIQAYDEQMSEYMNKRTNGKETRNWSQSIASYYERLYYPHIREMLEKQGF-

General information: TITO was launched using: RESULT: Template: 1ZCH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 964 -42252 -43.83 -179.03 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -43.83 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_1ZCH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZCH-query.scw PDB file : Tito_Scwrl_1ZCH.pdb: