Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKYQEETMKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTMFVTGQRSEERRVSI 4ZRB Chain:H ((4-129)) -------MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHGGYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMDVDITNQEGRNVCKATFTMFVTGQR--------

General information: TITO was launched using: RESULT: Template: 4ZRB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 631 -88169 -139.73 -699.75 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain H : 0.96 3D Compatibility (PKB) : -139.73 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.96 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_4ZRB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZRB-query.scw PDB file : Tito_Scwrl_4ZRB.pdb: