Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLGN-----VLFGDWKDVAPLLEGLVEN-QDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEIGDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAEPVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQTQQKTALEDALRTL 3CJ8 Chain:A ((5-221)) ------DAYEIIQYIGDAKKQTLVKVTLKGQLKEVTFPETIKVFNNCKTGTLFGDWADVKPFLEANKEKIEDYVVENDARNSAIPFLDLKDINARIEPGALIREKVEIGDQAVIMMGAILNIGAVVGAGTMIDMGAVLGGRATVGKHCHIGAGTVLAGVIEPPSAAPVVIENEVVIGANAVVLEGVRVGEGAVVAAGAVVVEDVPAHTVVAGVPAKVIKQIDD---------------

General information: TITO was launched using: RESULT: Template: 3CJ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1156 -94921 -82.11 -449.86 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.88 3D Compatibility (PKB) : -82.11 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.649

(partial model without unconserved sides chains): PDB file : Tito_3CJ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CJ8-query.scw PDB file : Tito_Scwrl_3CJ8.pdb: