Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEANMKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNSNNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSEGSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISSE--KVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAISTKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINIIEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIP------SNVTSGVIVRSVQSNMPAN-GHLEKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLES 5T69 Chain:A ((27-361)) ------------------------------------------------------------SESFVAAAVSRSGPAVVRIDTE-----------------------QGSGFIIDN---SGIILTNAHVVDGASKVVVTLRDGRTFDGQVRGTDEVTDLAVVKIEPQGSAL-PVAPLGTSSNLQVGDWAIAVGNPVGL--DNTVTLGIISTLG--------------VEFIQTDAAINPGNAGGPLLNARGEVIGINTAI---------TGIGFAIPIDQAKAIQNTLAAGGTVPHPYIGVQMMNITVDQA---QQNNRNPNSPFIIPEVDGILVMRVLPGTPAERAGIRRGDVIVAVDGTPISDGARLQRIVEQAGLNKALKLDLLR--RRLSLTVQTAQ-------

General information: TITO was launched using: RESULT: Template: 5T69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1518 -44265 -29.16 -162.14 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -29.16 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.568

(partial model without unconserved sides chains): PDB file : Tito_5T69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T69-query.scw PDB file : Tito_Scwrl_5T69.pdb: