Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHFTFLGTSSGVPTLSRNVSGLAVRNSK--NKDWILVDAGEGTQHRIQQARLSLQNLIAICITHVHGDHCYGLVGLLASAGMNARNNPLIVIAPKEIQQWFEITAQLTDLHLPYSIKFIDVNEATRPQQLTDELFIQAHPLSHRVPSFAFSIYIKSTQKKIDIQALTQLGIPKGDIWGHLKRGYDVEFE-GRILKSQDFIKIQNQQIHAIIGGDNDRPELLADACKDAQLLIHEATYLQTVLDKVGKGPMHSSAKMVAEFAEQQSLDNLILTHFSPRHQDKTGQQAIIEEVRQFYKGHFYLANDFDEFTLNETGQLLKIE 2CBN Chain:A ((1-306)) AMNLIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQHQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVETALRISGSWTDYPLEIVEIGA-G-EILDDGLRKVTAYPLEHPLECYGYRIEEHDAPGALNAQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDAALDLAKGVDVMVHEATLDITMEAKANS-RGHSSTRQAATLAREAGVGKLIITHVSSRYDDKGC-QHLLRECRSIFP-ATELANDFTVFNV----------

General information: TITO was launched using: RESULT: Template: 2CBN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1697 -21543 -12.69 -71.10 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -12.69 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_2CBN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CBN-query.scw PDB file : Tito_Scwrl_2CBN.pdb: