Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLFSGSSMSEQIFIQGPVGKIELFVDRPEGEI-KGFAVVCHPHPLQGGTPQHKVPALLTQIFNEYGCIVYRPSFRGLGGSEGVHDEGHGETEDILAVIEHVRKLHAGLPFYAGGFSFGSHVLAKCHAQLSPELQPVQLILCGLPTATVVGLRHYKTPEIQGDILLIHGEQDDITLLSDAIEWAKPQKHP--ITILSGANHFFTGYLKQLRQIITRFIIMK 3TRD Chain:A ((1-208)) ---SYVMTNEDFLIQGPVGQLEVMITRPKGIEKSVTGIICHPHPLHGGTMNNKVVTTLAKALDELGLKTVRFNFRGVGKSQGRYDNGVGEVEDLKAVLRWVEHHWSQDDIWLAGFSFGAYISAKVAYDQKVA----QLISVAPP---VFYEGFASLTQMASPWLIVQGDQDEVVPFEQVKAFVNQISSPVEFVVMSGASHFFHGRLIELRELLVRNLA--

General information: TITO was launched using: RESULT: Template: 3TRD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1225 -59457 -48.54 -290.03 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -48.54 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.630

(partial model without unconserved sides chains): PDB file : Tito_3TRD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TRD-query.scw PDB file : Tito_Scwrl_3TRD.pdb: