Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVVVFSQIDEEILSRLQQDYHVVVLNPKLGDINEQIRQEVVDADGMIGA-GRLLNESNLSPAQKLKIISSVTVGYDNYDVGYLNQKKIWLANTPHVLTETTADLAFTLLLSAARKVPFLDHWTKQGEWKR---TVGPQQF-GLDVFGKTLGIIGLGNIGAAIARRGFYGFNMNIVYHNRREKPELAEPLKAQYLGLEELLQQSDFVVTAVGLNAESKALMGKAQFELMQKHAIFINIARGSVVDEQALIEALQNEVIFAAGLDVYEKEPLQESALFNLPNVVTLPHVGSATAETRKKMANLAYKNLVEALEDKTPRYLVNPNFV 5AOW Chain:A ((4-322)) --KVLITRAIPENGIELLREHFEVEVWEHEHEIPREVLLEKVKDVDALVTMLSEKIDREVFDAAPRLRIVANYAVGYDNIDIEEATKRGIYVTNTPDVLTDATADLAWALLLAAARHVVKGDKFVRSGEWKRRGIAWHPKMFLGYDVYGKTIGIVGFGRIGQAIAKRA-KGFGMRILYTARSRKPEAEKELGAEFKPLEELLRESDFVVLAVPLTKETYHMINEERLRLMKPTAVLVNVARGKVVDTKALIRALKEGWIAAAGLDVFEEEPYYDEELFALDNVVLTPHIGSATFGAREGMAELVAKNLIAFKNGEVPPTLVN----

General information: TITO was launched using: RESULT: Template: 5AOW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1667 -505 -0.30 -1.61 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -0.30 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.552

(partial model without unconserved sides chains): PDB file : Tito_5AOW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AOW-query.scw PDB file : Tito_Scwrl_5AOW.pdb: