Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKPVPKIYRTTNWSSYNQALIKRGNISIWFD--PKTQWYAQPQGKHGRNQTYSDAAIQCC------------LMIKSLFRLSLRMVTGFAQSLIKLCGLNWTAPDYS-------TLCRRQKRIDIAISYQQSRDGLHLLVDSTG-----LKFLGEGEWKRKKHQPAYRRQWRKLHIGID---AKTLQIRAVQLTTNNVSDSQVLGDLLDQIPLDERIDSVYTEG-AY----DTKHCRQV---------------ILDRDAHAV---------------IPPRKNAKPWKDK-KLRSLERNELLKTVKRLGR--------SLWKKWSGYHRRSLVETKMHCIK-------- 4QNU Chain:A ((1-323)) ----------MIDFGNFYS-LIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSNAVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNL--MPWRKGPFSLYGVNIDTEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGA---------------HLAVGIDPTQLFLCQFEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNEGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVSVTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP

General information: TITO was launched using: RESULT: Template: 4QNU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1149 3326 2.89 13.74 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 2.89 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.254

(partial model without unconserved sides chains): PDB file : Tito_4QNU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QNU-query.scw PDB file : Tito_Scwrl_4QNU.pdb: