Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNEINYLTMAMSLIALAMSFLTFQCNLLIVGMCYGAALVLIIIYIILKINRKTRNKDSNKC 3TGL Chain:A ((135-157)) ------------------------SYKVAVTGHSLGGATVLLCALDL---------------

General information: TITO was launched using: RESULT: Template: 3TGL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 26 -8953 -344.33 -389.24 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -344.33 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.969

(partial model without unconserved sides chains): PDB file : Tito_3TGL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TGL-query.scw PDB file : Tito_Scwrl_3TGL.pdb: