Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLTKQQRAELKQKFGGHCAYCGDLLG-----------------------------------------DKWHADHIEAVKRDLIHVGGGKLITGEMTRPQN-DTLENMNPACVPCNKNKSSMPLEGWRKLLTHYRDVQLLRDSTHAHHLLRFGLIEIKTKPVTFFFENYKGASHE 5MKW Chain:A ((3-110)) -SNNSYLRAKVFETEHGVCQLCNVNAQELFLRLRDAPKSQRKNLLYATWTSKLPLEQLNEMIRNPGEGHFWQVDHIKPVY-----------------GGGGQCSLDNLQTLCTVCHKERTARQAKE-------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5MKW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 133 8835 66.42 133.86 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 66.42 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.226

(partial model without unconserved sides chains): PDB file : Tito_5MKW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MKW-query.scw PDB file : Tito_Scwrl_5MKW.pdb: