Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLEQLQRLQAHIGVLKTRLHHLESENSALSEAKELAETEHHAQVVQKNSIITKKQEEIETLTEQLTQLQGQFQQLNQNANTLAERYSRLEKSTTDLKNRFQEILAERNELRVTKEKLQSQQRQTQQELHDLQQDRDRLLQKNELAKAKVEAIIQRLAILGTAQDQHAQEIQQLAHPNAEAGEETQS 2EFR Chain:A ((149-286)) -----KQLEDKVEELLSKNYHLENEVARLKKLLERAEERAELSEGK----SAELEEELKTVTNNLKSLEAQAEKYSQKEDKYEEEIKVLSDKLKEAETRAEFAERSVTKLEK--------------SIDDLEDELYAQKLKYKAISEEMKQLEDKVEELLS-------------------------

General information: TITO was launched using: RESULT: Template: 2EFR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3 -962 -320.67 -6.97 target 2D structure prediction score : 0.93 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -320.67 2D Compatibility (Sec. Struct. Predict.) : 0.93 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.611

(partial model without unconserved sides chains): PDB file : Tito_2EFR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EFR-query.scw PDB file : Tito_Scwrl_2EFR.pdb: