Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVMLSLSVFFKGLGIGSGLIVAI--GAQNAFVLKQGLKQ-QYVFWLCLICALSDSILIACGVLGFAEIMTASPILITVAKYLGATFLFVYGAKAFYAAFKT-NQG--ME--LDSSQKQTLTQALITCLAFTW-LNPHVYLDTIVLIGSVATQLEDKISFALGSILASWIFFFSLGYGARFLKPLFINPKAWKILDFMIGCVMWSIAISLLI 2N4X Chain:A ((11-194)) --------QLMAFALGILSVFSPAVLPVVPLIFAGSRGRALDAFLIVAGLTISMLILGYTASLF-F----------GFFRVVAMLFLLIFALILLSDELDEKVSIFASRMTSGLSWKIQTLPSFFFGMLLAFLWLPAILPFAGIAISQTLLSEN--PLVMLSYGLGMAVTIAAVFKMGE---KFVK--ANFQLIRKVTGAIVLLYLAYFA-

General information: TITO was launched using: RESULT: Template: 2N4X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 603 -85522 -141.83 -488.69 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -141.83 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.149

(partial model without unconserved sides chains): PDB file : Tito_2N4X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2N4X-query.scw PDB file : Tito_Scwrl_2N4X.pdb: