Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALLRIEKNNGIATVYLNRPDKRNAMSFALLKELVTTAKAIQKDRDIRCVILTGEAQVFSAGIDLSDLNNPKNSAFAIWELVKPGQSLFQKAFLIWQNLPVPVIAALEGYCFGAGMQLALAADIRIAHPDTKMSIMESRWGLVPDMGLTRSLKGLIGVDLAKELTLTARVLDGNYAKDIGLVTHLSENPLEKANAIASEMLQRSPDALTAAKRVLD-AMEHQPEKSLRLEKIWQLKLLLGKNSKLARKKDKHPEVKFLPRQYR 4OG1 Chain:A ((40-277)) -----------VAHVLLDRADKMNALDDAMFEGIVAAGHHLHSVKGVRCVVLSGAGRSFCAGLDLS----------SLTERTHGNANRAQQAAMVWRKLPMPVIAAVHGVCFGGGLQVASGADIRFITPDARLAVMEVKWGLVPDMAGYALWRGNVRDDVLRELTYTHREFSGEEAVRFGFATHVADDPLAGAMELARVVAEKSPNAVRGAKTLSNRAPDLTVDDVLMAESIAQHELMYSRNQMEAVK---------------

General information: TITO was launched using: RESULT: Template: 4OG1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1168 -146384 -125.33 -647.71 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -125.33 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_4OG1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OG1-query.scw PDB file : Tito_Scwrl_4OG1.pdb: