Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNFDRNTPIAFLGIGLMGNRMASHLIQAGFQVAVWNRTASACEELIDIGAHALDLS--NIGQYPLILTCLADDKAVQAVFD--QIQTNLKAGQVIVDFSSLSVAATKALAQAAALQDVIWIDSPVSGGTAGAEQGTLVIFAGGDAQTIEALSPVYNVLSQRVTRMGDTGTGQATKICNQLIVAANSALIAEAVALADRAGVDTTLLAPALAGGFADSKPFQILAPRMATHTFEPVQWKVQTLSKDLNNAVTLANNVNLDIPVAQKALLQLQTHQKNSFAEKDLATMIQVVEQ 4DLL Chain:B ((32-315)) ------RKITFLGT--MGLPMARRLCEAGYALQVWNRTPARAASLAALGATIHEQARAAARDADIVVSMLENGAVVQDVLFAQGVAAAMKPGSLFLDMASITPREARDHAARLGALGIAHLDTPVSGGTVGAEQGTLVIMAGGKPADFERSLPLLKVFGR-ATHVGPHGSGQLTKLANQMIVGITIGAVAEALLFATKGGADMAKVKEAITGGFADSRVLQLHGQRMVERDFAPR-ARLSIQLKDMRNALATAQEIGFDAPITGLFEQLYAEGVEHGLTDLDQSGLFVELAS

General information: TITO was launched using: RESULT: Template: 4DLL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1476 -79472 -53.84 -285.87 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -53.84 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_4DLL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DLL-query.scw PDB file : Tito_Scwrl_4DLL.pdb: