Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTSNKDVQACLKVVHDALVDVKAKDILQLDVSSISNVADAIVIASGTSTRHVKALADNVAEEARKAGFRPIGVEGERDAEWILIDLGFVVVHVMLPTARKFYDLESLWRTAPESVA 4WCW Chain:D ((3-114)) ---ANREAIDMARVAAGAAAAKLADDVVVIDVSGQLVITDCFVIASGSNERQVNAIVDEVEEKMRQAGYRPARREGAREGRWTLLDYRDIVVHIQHQDDRNFAALDRLWGDCPVV--

General information: TITO was launched using: RESULT: Template: 4WCW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 560 -87908 -156.98 -784.89 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.75 3D Compatibility (PKB) : -156.98 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.677

(partial model without unconserved sides chains): PDB file : Tito_4WCW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WCW-query.scw PDB file : Tito_Scwrl_4WCW.pdb: