Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILITGANTGIGFATAEQLVKQGQHVILACRNPQKAQEAQNKLRSLDQGQVDVASLDLNSLELTQKAAEEIADKYGSLDVLINNAGLFSKTKQLTVDGFEQKFGVNYLGHFLLTQKLLPVLKQFPQAHIIHLASIAHWVGSIKPNKFRA-EGFYNPLFYYGQSKLANLLFSNALAEQLAD--SSITNNALHPGGVASDIYRDLPKPVYAAMKVGLVPTSV--PAKLITEMATGDTWQ----NRNGEYVSAHMPDWKSSHAKNQQLARDLYQQSMNLVEKFL 3RD5 Chain:A ((17-288)) RTVVITGANSGLGAVTARELARRGATVIMAVRDTRKGEAAARTM----AGQVEVRELDLQDLSSVRRFADGV----SGADVLINNAGIMAVPYALTVDGFESQIGTNHLGHFALTNLLLPRLT----DRVVTVSSMAHWPGRINLEDLNWRSRRYSPWLAYSQSKLANLLFTSELQRRLTAAGSPLRALAAHPGYSHTNL-----------------ATDADFGARQTLYAASQDLPGDSFVGPRFGYLGRTQPVGRSRRAKDAGMAAALWALSEQLTKTE-

General information: TITO was launched using: RESULT: Template: 3RD5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1443 -68912 -47.76 -283.59 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -47.76 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.549

(partial model without unconserved sides chains): PDB file : Tito_3RD5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3RD5-query.scw PDB file : Tito_Scwrl_3RD5.pdb: