Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIYKVYLNIISLHWGRTDMNLSYLKTSLYTAATTAILLCSSQSQAQQYYKWMDKSGSTHYTTTPP------PKGAKHLNKVSTYGSQPLLKNPTSNSEQPSQDKDKVVQEVTNVAVEKGAPAVPVPPAPSVSAPR 2NR6 Chain:D ((74-142)) -----------------TSSNTAYLHLNSLTSEDTAVYFCARWVRQMDYWGQGTSVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLG--------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2NR6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 73 -6992 -95.77 -110.98 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain D : 0.61 3D Compatibility (PKB) : -95.77 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.176

(partial model without unconserved sides chains): PDB file : Tito_2NR6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NR6-query.scw PDB file : Tito_Scwrl_2NR6.pdb: