Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIVIAMGVCGTGKTLIGELLSERLACEFLDGDTLHSAANKSKMSQGIPLTDEDRLPWLQAIRQAIEAKQRDGETAVFTCSSLKRMYRDILRGQDQNVKFVYLKGSYELLQQRLAERSGHFFDPALLQNQLDTLE--EPDVNEAIAIDIALTPEQIIEQVIQKLGVTDSVCRG 4EUN Chain:A ((32-198)) --VVVMGVSGSGKTTIAHGVADETGLEFAEADAFHSPENIATMQRGIPLTDEDRWPWLRSLAEWMDARADAGVSTIITCSALKRTYRDVLREGPPSVDFLHLDGPAEVIKGRMSKREGHFMPASLLQSQLATLEALEPD-ESGIVLDLRQPPEQLIERALTWLDIAPAVA--

General information: TITO was launched using: RESULT: Template: 4EUN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 749 -75634 -100.98 -458.38 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -100.98 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_4EUN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EUN-query.scw PDB file : Tito_Scwrl_4EUN.pdb: