Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLVYTLIAIVALIVMIAKFRIYPFLVLIIVSLGLALAVGMPMDGIVKSYEAGTGKTLGHLAIVIALGSMLGKMMAESGGAERIAIALIKWFGEKHIHWAMMFIALIVGLPVFFEVGFVLLIPIAFNIAKRTGKSLLIVGLPMVAG--LSVVHGLIPPHPAALL--AV-QAY-------HADIGKTIMYSLLVGVPTAVVAGPLYALWINKYVKLPENNPLAKQFVEADANNTRELPSFGITLFTIMLPVALMLVGSWADVFFAPKTFPNELLRFIGTSD---IALLIAVLVSFITFGTMQ---GF-NREQIEKFCGGCLASIAGILLIVGAGGGFGGILRDSGISNEIVSTALKA-N---LSPLL---LGWLVAAIIRLATGSATVAMATACSIVAPIAAAAGVVVRPELLVLATGSGS---LIFSHVNDAGFWLIKEYF-G----MTVGQTLKTWSVLETIISVLGLSFTLLLSAVL 4R0C Chain:C ((64-484)) ----------------------------------------DGLRYLFTTLVGNFTGF-APLG-VVLVAMLGLGVAEQSGLLSVSLASLVRRSSGGALV-FTVAFAGVLSSL-TVDAGYVVLIPLAGLVFQLAGRPPIAGIATAFAAVSGGFSANLLVGPVDATLAGLSTEAAHIIDPDRTVAATGNYWFIIASTFLVTGL-VTLITRTLTEPRLAHANTVADAS-VDAPQIHSRAMKWTGLTLAILLAGLALLVLPND-APLRHPDTG--SV---LGSPFIHGLVVIVALIAGICGAVYGRVSGQFRNSGAVITAMEVTMASMAGYLVLMFFAAQFVAWFNYSQLGLLLAVKGAAWLGALTVPKVVLLLLFVVLTALINLMIGSASAKWSILAPVFIPMLMLLG--ISPEASQAAYRVGDSSTNIITPLMPY-FVLVLGFARRYQPETGIGTLIALMLPYSLTLLLGWSVLLGVWI---

General information: TITO was launched using: RESULT: Template: 4R0C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1878 -200464 -106.74 -517.99 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -106.74 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.366

(partial model without unconserved sides chains): PDB file : Tito_4R0C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R0C-query.scw PDB file : Tito_Scwrl_4R0C.pdb: