Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYVPTIENALVPVVVEQSSRGERSFDIYSRLLRERVIFLTGEVEDNMANLIVAQMLFLEAENPDKDIHLYINSPGGSVTAGMAIYDTMQFIKPDVVTYCMGQAASMGAFLLNAGAKGKRYCLENARVMIHQPLGGFRGQASDIEIHAREILFIKERLNRLMAEHSGQDYDTIARDTDRDNFMTAQAAKEYGLVDQVLSKRP 2FZS Chain:E ((2-192)) ---------LVPMVIEQ----ERSFDIYSRLLKERVIFLTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVSTICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVKGRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHR-

General information: TITO was launched using: RESULT: Template: 2FZS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 853 -136692 -160.25 -730.97 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain E : 0.92 3D Compatibility (PKB) : -160.25 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.92 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_2FZS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FZS-query.scw PDB file : Tito_Scwrl_2FZS.pdb: