Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLIEQLQSKIEQARTLYKEFRLYDLGSFALNRFTPKDGFEQVLNVRYGLKPRHRLDIYRSTKRLAHRPLIVFVHGGAWQHGNKRDYLFIGEAFTKEGYDVAVINYQLAPKNIFPSYVDDLTQALNYLHQNQEKLEISTENIVLMGHSAGAFNVMSAVYHPKPNTIQCLGNIKAIFGLAGPYHFDYKGDPLAEDAFDQSI------SYKEVMPYYF-----VNQNHIKHYLLMAENDQLVKKENTFDLDQALRQSGNHSHIAVIPKTGHITILASLS---SFVSHYFRTKRTILHFLDEVF 4E11 Chain:A ((50-298)) ---------------------------------LTEKQGI-TVDHLRYG-EGRQLVDVFYSEKTTNQAPLFVFVHGGYWQEMDMSMSCSIVGPLVRRGYRVAVMDYNLCPQVTLEQLMTQFTHFLNWIFDYTEMTKVSS--LTFAGHSAGAHLLAQILMRPNVITAQRSKMVWALIFLCGVY--DLRELSNLESVNPKNILGLNERNIESVSPMLWEYTDVTVWNSTKIYVVAAEHDSTTFIEQSRHYADVLRKKGYKASFTLFKGYDHFDIIEETAIDDSDVSRFLR-------------

General information: TITO was launched using: RESULT: Template: 4E11.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1241 -149491 -120.46 -636.13 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -120.46 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_4E11.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4E11-query.scw PDB file : Tito_Scwrl_4E11.pdb: