Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQTITLYVDGACRGNPGLGGWGAYVITEQGEHKLFGGEPDTTNNRMELTAAIEGISFCPPDAQLIVWTDSNYVKQGITEWIHGWKKKNWKDVKNPDLWQKLDAVCAGRNIEWNWIKGHAGHAGNEMADQLANLGADKTAQELKQPQSATVSQDIKKPEQDWLLDDPFGFDLVETTEDETITLEQTQEVEMIVVDEEMNVVEAVEQEEQQQNTNIHPQIVMTEAKLKLQGPRQLILDTETTGF----YFQDGDRIIEVGAIEMINRKLTGSSIHIYINPEKPVG-DSVQVHGITDEFLVDKPKYAEIADTLFEYLKGAEIIAHNATFDMNFLDMEFK--RVGLPALSEVCEVTDTLALAKNKHPGQKNSLDALVRRYEIPARDRTFHGALLDAEILSDVYLAMTGGQVSFDMDALSQSESDQQKTTKARV-QIELPVIYPSDEELNIHETWVKEFEKKHGEPCLFAK 5FKU Chain:D ((7-240)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------RQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLTGNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAFDIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRTLHGALLDAQILAEVYLAMTGGQLSLPLA-----------------QASKLRVVFATDEEIAAHEARLDLVQKKGG-SCL---

General information: TITO was launched using: RESULT: Template: 5FKU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 796 16066 20.18 77.61 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain D : 0.71 3D Compatibility (PKB) : 20.18 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_5FKU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FKU-query.scw PDB file : Tito_Scwrl_5FKU.pdb: