Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSITLLQPDDWHAHLRDGLALKRTVPDLAKQFARAICMPNLVPPVKTVEEALAYRERILAHVPEGNNFDPRMVLYFTDHTSPDEVRKIKESEHVNAIKLYPAGATTNSNNGVSDIRKVYAVIEQLEEHQVPLLLHGEVTHNHVDIFDREKRFLDEVLSPLLKQFPKLKVVLEHITTSDAAHFVLEQDRNVAATITPQHLLFNRNDMLVGGIKPYFYCLPILKRQTHQTTLLEVATSGNPKFFLGTDSAPHAQNAKENACGCAGCYSAPNAIELYAQAFDQVGKLERLEGFASHFGADFYGLPRNTSTITLVKEDNLVPESFDYLDNQKIIPLHAGKTLQWRKV 5VGM Chain:A ((4-342)) MTTLTITRPDDWHVHLRDGDVLADTVRDISRYNGRALIMPNTVPPVTTTEMALAYRERIMAAQ---H-FEPLMALYLTDNTSPEEIRKAKASGKVVAA-LYP---------GVTSAKNIYPVLQAMQEVGMLLLVHGEVTTHEVDIFDREKTFLDTVLAPIVNDFPQLKIVLEHITTADAVTFVQQAGDNVAATITAHHLLFNRNHMLVGGIRPHFYCLPILKRATHQHALVAAATSGSKKFFLGTDSAPHAKGRKEAA-G-AGSYTAHAALELYAEVFEKEGKLENLEAFASFNGPDFYGLPRNQETVTLTKQAWPVAESMPF-GSDIVVPIRAGENIEW---

General information: TITO was launched using: RESULT: Template: 5VGM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1843 -186195 -101.03 -574.68 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -101.03 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_5VGM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VGM-query.scw PDB file : Tito_Scwrl_5VGM.pdb: