Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKYFELLDWVTAEKFCELTGEQVSNLKNLR-PQWDEGKVWVKVSDR-------KILYSLKGYNAWVEQAAQVYQKAQEQDREKYRLTLNGTTSASGSPSTSSIPQPTSLKRLRLEKIL 2KVV Chain:A ((1-78)) MAQIIFNEEWMVEKALMVRTGLGARQIESYRQGAWIEGVHFKRVSPSGEKTLRGTTWYNYPEINKFIRDSLEHHHHHH-----------------------------------------

General information: TITO was launched using: RESULT: Template: 2KVV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 184 -13113 -71.27 -187.33 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -71.27 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_2KVV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2KVV-query.scw PDB file : Tito_Scwrl_2KVV.pdb: