Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKNNSRQFINGQRVAVNVPALESIAAKDYEPTGYVFSQATLEEVDQAAQAAHNAFLVYSQTTQEQRASFLEEIARQIEALGANLQEVASLETGLPLARLQGETGRVTGQLRLFAELLRRGDFYGARIDVALPERKPLPRVDLRQYKVGVGPVAVFGASNFPLAFSTAGGDTVAALAAGCPVVFKAHSGHMATAELVAEAIEKAIEVCGMPKGTFNMIFGGR--IGANLVEHSLIQAAGFTGSLEGGMALYNLAQSRPQPIPFFAEMSSVNPVVVLPEALHSRGEQIAQDTVASFNMGCGQFCTKPGLILGVKGPEFDRFITALAENTQKAVPQVMLNEGTLKSYRAGIDALINEAGFEIIASGQEPELVSQAKAHLFKADQSVLLSGNPKLQHEVFGPMSIVIAVEDEATLLKGIEALGGQLTATIIGDENELSKAGELLDLLTRKAGRVLFNGFPTGVEVSDAMVHGGPFPATSDARGTSVGTGAIERFLRPVCFQNTPQVLLPDALKDSNPLQITRLVNGQLTQAQI 1EYY Chain:B ((24-506)) ------------------------------------FPVHTEVEVNQAATAAAKVARDFRRLNNSKRASLLRTIASELEARSDDIIARAHLETALPEVRLTGEIARTANQLRLFADVVNSGSYHQAILDTPNPTRAPLPKPDIRRQQIALGPVAVFGASNFPLAFSAAGGDTASALAAGCPVIVKGHTAHPGTSQIVAECIEQALKQEQLPQAIFTLLQGNQRALGQALVSHPEIKAVGFTGSVGGGRALFNLAHERPEPIPFYGELGAINPTFIFPSAMRAKAD-LADQFVASMTMGCGQFCTKPGVVFALNTPETQAFIETAQSLIRQQSPSTLLTPGIRDSYQSQVVSRGSDDGIDVTFSQAESPCVASA---LF-VTSSENWRKHPAWEEEIFGPQSLIVVCENVADMLSLSEMLAGSLTATIHATEEDYPQVSQLIPRLEEIAGRLVFNGWPTGVEVGYAMVHGGPYPASTHSASTSVGAEAIHRWLRPVAYQALPESLLPDSLKAENPLEIARAVDGK------

General information: TITO was launched using: RESULT: Template: 1EYY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2843 -63949 -22.49 -132.95 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -22.49 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_1EYY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EYY-query.scw PDB file : Tito_Scwrl_1EYY.pdb: