Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAQMKTNASAKKAVNSPNHIYDTFIVGAGISGIAAAIRLDQVGYTNYKIIEKAGRVGGTWRENTYPGCGCDVPSALYSYSFAPSA----KWGHLFARQPEILSYLEDVSNEFNITSKIEFYNELLNAAWDESRHLWVLDTTTG-QYLSRTVIFATGPITEAQIPRLEGLDTFTGEMFHSAKWNHDY-DLTGKRIAVIGTGASAIQFVPQIQPKAKELFVFQRTAPWVLPKPDTDLGELSKSII---------------------------------AKYPAIQASWRKSVALTLNAINFGLRNPLALKPVNVLGKQLLKLQIADPELRKNVTP-NFDIGCKRILFANNYYPALQAPNTTLIPHGLVKVEG--NTVIAANGERHEVDVIIWGTGFEVSHPPIGK-RVFNEKGQRLNDLWKNSSPEAYLGTNIENVPNAFLVLGPNV-LVYDSFIGLAEAQLDYIVDGLLKIKNKGISKLNVKADVIKKHNDLVQKHLQTTVFNSGGCKSYYLDAN----GRNFAAWPWSLKKLKQRLKKLDLKDYEVTYQMSKTH 1W4X Chain:A ((10-542)) --------------RRQPPEEVDVLVVGAGFSGLYALYRLRELGR-SVHVIETAGDVGGVWYWNRYPGARCDIESIEYCYSFSEEVLQEWNWTERYASQPEILRYINFVADKFDLRSGITFHTTVTAAAFDEATNTWTVDTNHGDRIRARYLIMASGQLSVPQLPNFPGLKDFAGNLYHTGNWPHEPVDFSGQRVGVIGTGSSGIQVSPQIAKQAAELFVFQRTPHFAVPARNAPLDPEFLADLKKRYAEFREESRNTPGGTHRYQGPKSALEVSDEELVETLERYWQEGGPDILAAYRDILRDRDANERVAEFIRNKIRNTVRDPEVAERLVPKGYPFGTKRLILEIDYYEMFNRDNVHLVDTLSAPIETITPRGVRTSEREYELDSLVLATGFDALTGALFKIDIRGVGNVALKEKWAAG-PRTYLGLSTAGFPNLFFIAGPGSPSALSNMLVSIEQHVEWVTDHIAYMFKNGLTRSEAVLEKEDEWVEHVNEIADETLYPM--TASWYTGANVPGKPRVFMLYVGGFHRYRQICDEVAAKGYE-GFVLT---

General information: TITO was launched using: RESULT: Template: 1W4X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2692 -2316 -0.86 -4.78 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -0.86 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_1W4X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1W4X-query.scw PDB file : Tito_Scwrl_1W4X.pdb: