Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKGSFMPASTSQYYTRTAQILHWVMAIIFITAWVIGFYSGNFL-SYD-----------VDGSFKGDVITLHKNIATTIIFLVVIRLFWRYTHPAPQLPDTMSPTMKTLAHIGHLLLYVILVALPVTGCLFSWSAGHPA------PVLYLFEIPRL-----------VQDNPELLAIVKPLH-IYISWFAGFLIVGHVLAALKHHFVDKDNVLNSMTKQPK 4H13 Chain:A ((39-207)) -------------------------ITLTCFLIQFATGFAMTFYYKPTVTEAYASVQYIMNEVSFGWLIRSIHRWSASMMVLMMILHVFRVYLTGGFKKPRE-------LTWISGVILAVITVSFGVTGYSLPWDQVGYWAVKIVSGVPE--AIPVVGVLISDLLRGGSSVGQATLTRYYSAHTFVLPWLIAVFMLLHFLMIR-------------------

General information: TITO was launched using: RESULT: Template: 4H13.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 367 -54903 -149.60 -394.99 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -149.60 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.192

(partial model without unconserved sides chains): PDB file : Tito_4H13.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4H13-query.scw PDB file : Tito_Scwrl_4H13.pdb: