Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTELTPYHEDQELDNNTSDNIHFRDILEQHISRRSLITKAASGAVALTLASTLTGCNDNDDDSGSNNGGTTPVDPNKKPEKLTFTPVAKNLNDIVTVPEGYEANVIYALGDSINPRVGDWDDNNIPSGPSFQFRSGDCHDGMHYFGLNTSTNRFDETVSAQGLLVMNHEYINQTFLHPKGPTKVDGRRPEDEVIRETNANGVSVIHIKKDPTTQKVEIIKNSIFNRRITASTVMEFAGAAAGSSLLATRFSPAGRQTRGTHNNCGNGYTPWGTYLTTEENFIGYFQRSGSDEYARTDAEKITLKRYGLGVKKDEPYLYEKDEKGAPKKDKDGKIIYLKDKNGELIPNVDEQGRQIYLGTSSRYGWETAIGQVESQDLYDRWNADVKAAQATQDYRNGPNTFGWMVEIDPFDGRQNPVKRTSLGRFAHEDSACRAVVGQPLAFYMGDDSRGEYIYKFVSTAVWDTKDINGGYTAGDKYMNAGKLYVAKFNN--------DGSGQWIELAYGKNGLNESNTTYPFKSQADVVTFARLAADSVGATKMDRPEWCTINPLNGEIYVTLTNNSNRGKD-YATDAANPRNYTDLYAGTKEQKGNVNGHIIRFKETDDKTTAETFKWDIYLFGAEAAMASNINLSG---LTDNNDFSSPDGMWFDPRGVLWIETDDGAYTDVTNCMMLAALPGQIGDGGTATTSNGQQTITGAKVTDATLRRFLVGPKQCEITGIAMTPDYKAIFINVQHPGEDSPSYAKPESNWPATQKDPSNKTARPRSATVVITRKDGGVIAG-- 3ZWU Chain:A ((9-588)) -------------------------------------------------------------------------------RLLGFDSIPAATTDTISLPKGYKSSVLISWGQPLHKNGPAFDPSGNGTAAAQEVQFGDNNDGMSLFEFPG--------EKNRALMAINNEYTNYRYLYPHGGM----PQSAEDVRKALACEGVSVIEVQRKN--GQWQFVQGSRYNRRIHGNSPLRISGPAAGHELMKTSADKHGKKVLGTFQNCANGKTPWGTYLTCEENFTDCFGSSNAQ-----QQFDPAQKRYGVS------------------------------------------------AASREINWHPF---------DPRFDM--------AKNPNELNRHGWVVEIDPFDPQSTPVKRTALGRFKHENAALAETDDGRAVVYMGDDERGEFIYKFVSRDKINHRN----AKANRDILDHGTLYVARFDAGDGNPDHPKGQGQWIELTHGKNGIDASS---GFADQAEVLIHARLAASVVGATRMDRPEWIVVSPKDGQVYCTLTNNAKRGEDGQPVGGPNPR------------EKNVYGQILRWRTDRDDHASKTFAWDLFVVAGNPSVHAGTPKGGSSNITPQNMFNSPDGLGFDKAGRLWILTDGD------------------SSNAGDFAGMGNNQMLCADPATGEIRRFMVGPIGCEVTGISFSPDQKTLFVGIQHPGENGGS------TFPEHLPNG-----KPRSSVMAITREDGGIVGAHH

General information: TITO was launched using: RESULT: Template: 3ZWU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3827 66208 17.30 116.97 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : 17.30 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.427

(partial model without unconserved sides chains): PDB file : Tito_3ZWU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZWU-query.scw PDB file : Tito_Scwrl_3ZWU.pdb: