Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNISTLCQMSLLSFMLL--------NINGCASHPRQSVDLQEYLKNFIGKSSTTIQQ-----DLNLRSLGFQVARTPQKTSDQLIYTILRPLSIPIPIVSNVDMRGGSVPIQSGNLSGNSYDINFNCKVIFKLKNDIAESIQYEGKAC 5DUQ Chain:A ((50-134)) MAFSPFSIASLLTQVLLGAGENTKTNLESILSYPKDFTCVHQALKGFTTKGVTSVSQIFHSPDLAIRDTFVNASRTLYSSSPRVL---------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5DUQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 134 -6576 -49.07 -91.33 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -49.07 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.331

(partial model without unconserved sides chains): PDB file : Tito_5DUQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DUQ-query.scw PDB file : Tito_Scwrl_5DUQ.pdb: