Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPKSFLIYKNDKLDLCKTLAIIEERLELPSPMEQDDMAGKTLYDKLWDDHVVT--QRDDGSCLLYIDRHLLHEVTSPQAFEGLQLAG-RQPWRLSANVATPDHNVPTSKKERDQGIAGIEDDTSRIQVQTLDDNCKAFNIV-EFGINDIRQGIVHVVGPEQGLTLPGMTVVCGDSHTATHGAFGCLAHGIGTSEVEHVLATQCLVQKKSKNMLVRVDGVLGKGVTPKDVVLAIIGKIGTAGGTGYAIEFGGQVFRDMSIEGRMTVCNMAIEAGARVGMVAVDDKTIEYVKGRSYAPKGEQWDQAVAYWNTLHSDDDAVFDAVVELNGAEIEPQVSWGTSPEMVIPVSKAVPTLEQAKDDVQRNDWTRAYQYMGLNAGQALADIQLGRVFIGSCTNSRIEDIRAAAEVVK-GRKVAPSIKQAMIVPGSGLVKQQAEKEGLDKIFLEAGFEWREPGCSMCLAMNADKLQPGEHCASTSNRNFEGRQG-NGGRTHLVSPAMAVAAAIAGHFVDIRSF 4KP1 Chain:A ((23-445)) ---------------------------------------GMTIVEKILAKASGKKEVSPGDIVMANIDVAMVHDITGPLTVNTLKEYGIEKVWNPEKIVILFDHQVPADSIKA-----------AEN-HILMRKFVKEQGIKYFYDI---REGVCHQVLPEKGHVAPGEVVVGADSHTCTHGAFGAFATGIGSTDMAHVFATGKLWFKVPETIYFNITGDLQPYVTSKDVILSIIGEVGVDGATYKACQFGGETVKKMSIASRMTMTNMAIEMGGKTGIIEPDEKTIQYVKEAMKKHG------TERPFEVIKGDEDAEFAEVYEIEADKIEPVFACPHNVDNVKQAR-------------------------------EVAGKPIDQVFIGSCTNGRLEDLRMAIKIIEKHGGIADDVR-VVVTPASREEYLKALKEGIIEKFLKYGCVVTNPSCSACMGSLYGVLGPGEVCVSTSNRNFRGRQGSLEAEIYLASPITAAACAVKGELVDPRDL

General information: TITO was launched using: RESULT: Template: 4KP1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2668 -11668 -4.37 -27.98 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -4.37 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_4KP1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KP1-query.scw PDB file : Tito_Scwrl_4KP1.pdb: