Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKNASSPGKRFMKLAGMTASIATKTVSNSIRNLTADEEQKLAAKTKLFQDIGLQIADTLGEMKGAVMKVGQIASQYKDIF-PPEVAKAISKLQRQAPAMPFSAIQQQVERELGKPLNAAFKSFDEQPFAAASIGQVHKAVLPNGQQVVVKVQYPGVDEACESDLKQVRLALRLMGVLKIDKKLQDQLFAE-----IQDSLSAELNYEIEAQNLEVFKTFHSKLDDKIIIPTVYKDYSSRRILTLSLEQGDSIETASSWPIEIRNTIGRRLIRALGQEMFFLKRFHCDPHPGNFAFR-QDGSVIIYDYGSVKTLSNEIVYSFKRLVNAARHEDIDLIETELLELHSL-AEKGKFPSDLYKLWIEVLLRPLTT--TYDFAENSSQHDGMLLVKKSLKYWDVFKPSPDTLMVNRTISGHYWNLIHLKVHDNLNDLFKELVPLSN 4PED Chain:A ((8-381)) ------------------------------------------------------RIVRTLCKVRGAALKLGQMLSIQDDAFINPHLAKIFERVRQSADFMPLKQMMKTLNNDLGPNWRDKLEYFEERPFAAASIGQVHLARMKGGREVAMKIQYPGVAQSINSDVNN------LMAVLNMSNMLPEGLFPEHLIDVLRRELALECDYQREAACARKFRDL-LKGHPFFYVPEIVDELCSPHVLTTELVSGFPLDQAEGLSQEIRNEICYNILVLCLRELFEFHFMQTDPNWSNFFYDPQQHKVALLDFGATREYDRSFTDLYIQIIRAAADRDRETVRAKSIEMKFLTGYEVKVMEDAHLDAILILGEAFASDEPFDFGTQSTTEKIHNLIPVMLRHRLV-PPPEETYSLHRKMGGSFLICSKLKARFPCKAMFEE------

General information: TITO was launched using: RESULT: Template: 4PED.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1914 20891 10.91 57.39 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 10.91 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_4PED.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PED-query.scw PDB file : Tito_Scwrl_4PED.pdb: