Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIAALHQPSQVQLNQDGNLKHFLTIEGLSKENLTKILDTAQSFLDDNNNLINRPLLEGRTVMNLFFENSTRTRTTFEAAAKRLSANVLNIDIARSSTSKGETLRDTLWNLEAMAADIFVVRHSSSGAAHFIAKDVCPKVAIINAGDGRHAHPTQAMLDMLTIRREMKKPFEDLSVAIIGDIKHSRVARSDVAALQTLGCKDIRVIAPNTLLPVGFSEYGDHVRLFNNMDEGITGCDVIIALRIQNERIDSPALSSQSEFYRMYGLNKERLSLAKPDCIVMHPGPMNRGVEIDSSIADGEQSVILKQVTNGIAVRMAVLALSMQGQLQEQGLIDAIAL 3R7F Chain:A ((1-291)) -------------------MKHLTTMSELSTEEIKDLLQTAQELKSGK----TDNQLTGKFAANLFFEPSTRTRFSFEVAEKKLGMNVLNLDGTSTSVQKGETLYDTIRTLESIGVDVCVIRHSEDE--YYEELVSQVNIPILNAGDGCGQHPTQSLLDLMTIYEE-FNTFKGLTVSIHGDIKHSRVARSNAEVLTRLGA-RVLFSGPSEWQDEEN-----TFGTYVSMDEAVESSDVVMLLRIQNERHQSA--VSQEGYLNKYGLTVERAERMKRHAIIMHPAPVNRGVEIDDSLVESEKSRIFKQMKNGVFIRMAVIQRALQT-------------

General information: TITO was launched using: RESULT: Template: 3R7F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1614 -172661 -106.98 -593.34 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -106.98 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.640

(partial model without unconserved sides chains): PDB file : Tito_3R7F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R7F-query.scw PDB file : Tito_Scwrl_3R7F.pdb: