Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATLNPRDVVIVDGVRSAMGKSKNGMFRNVRADSLSAELVRALVARN-QFDVNEVEDLIWGCVNQTLEQGMNIGRNIGLLAGLPKTVAGQTVNRLCGSSMQAIHTAAAQIATNQGDIFIIGGVEHMGHVGMMHGIDLNPEASKHYAKASNMMGLTAEMLGRMNGITREEQDAFGVESHRRAWAATQEGRFKNEIIGVEGHDANGFKILCDIDEVIRPDANLEAFKALKPVFDPKGGSVTAATSSALSDGASAMLLMSAERAQALGLKPRAVIRSMAVAGCDAAIMGYGPVPATQKALKRAGLSIADIQTVELNEAFAAQGLSVLKGLGLYDK-QDIVNLNGGAIALGHPLGCSGARITTTLLNVMEQQDTQIGLATMCIGLGQGIATVIERV 1WDK Chain:D ((1-390)) --SLNPRDVVIVDFGRTPMGRSKGGMHRNTRAEDMSAHLISKVLERNSKVDPGEVEDVIWGCVNQTLEQGWNIARMASLMTQIPHTSAAQTVSRLCGSSMSALHTAAQAIMTGNGDVFVVGGVEHMGHVSMMHGVDPNPHMSLYAAKASGMMGLTAEMLGKMHGISREQQDAFAVRSHQLAHKATVEGKFKDEIIPMQGYDENGFLKIFDYDETIRPDTTLESLAALKPAFNPKGGTVTAGTSSQITDGASCMIVMSAQRAKDLGLEPLAVIRSMAVAGVDPAIMGYGPVPATQKALKRAGLNMADIDFIELNEAFAAQALPVLKDLKVLDKMNEKVNLHGGAIALGHPFGCSGARISGTLLNVMKQNGGTFGLSTMCIGLGQGIATVFERV

General information: TITO was launched using: RESULT: Template: 1WDK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2650 -68521 -25.86 -176.60 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain D : 0.91 3D Compatibility (PKB) : -25.86 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.440

(partial model without unconserved sides chains): PDB file : Tito_1WDK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WDK-query.scw PDB file : Tito_Scwrl_1WDK.pdb: