Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFFTQFFGALNDNVYKQALLLVITYGWIQQQSAPVSTLNNLAALLFILPYFFFSATAGQIADKYERSQLVRGIKILEIIIMLIGSAGFLLGHLWLLLLALFLMGTHSTFFGPIKYAILPEILKPHELMSGNALFQSGTSMAILFGMILGGAVIASSDGNLLWISITVVAIACIGYLFS-RFILPQKFAAPDLDIDWNFIRTSFQTIKYAKSLPLVFTILLGNSWYWFYGATYLTQIPQLTQQNLHASENVVSLLLTFFSVGIGVGSLLCRKIGGSEINIKMVPIGAVGLTVFALYLAASLAFVPERTGALLTLKDVFTQGWVYYHVMIAVTLLGISGGFYIVPLYAMMQAYSPRSHRARVVAANNILNAVFMVSSAIFSILILSVLKIDIKILFIITAVLSAIFSIWLLARLKPMLATAHLSLED 4ZP0 Chain:A ((16-368)) --LYEFSTYIGNDMIQPGMLAVVEQ-YQ-----AGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAI---LLAQNIEQFTLLRFLQGISLCFIGAVGYAAIRESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLP--WEGMFVLFAALAAISFFGLQRAMPETATRIGEKL----SLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVIS----------------SHAYLWMTAGLSIYAFGIGLANAGLV-RLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGG--------------------------------------

General information: TITO was launched using: RESULT: Template: 4ZP0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1690 -243023 -143.80 -690.40 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -143.80 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_4ZP0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZP0-query.scw PDB file : Tito_Scwrl_4ZP0.pdb: