Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLVTAIVKPFKLDDVREALSDIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIEIAISDEMVDAVIESITRVASTGKIGDGKIFLTNLEQVIRIRTGETGPDAV 4CO1 Chain:A ((1-112)) MKLVMAIIKPFKLDEVREALTSLGIQGLTVSEVKGFGRQKGQTEIYRGAEYSVSFLPKVKVEVAVSDDQYEQVVEAIQKAANTGRIGDGKIFVLDIAQAVRIRTGETNTEAL

General information: TITO was launched using: RESULT: Template: 4CO1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 440 -59929 -136.20 -535.08 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -136.20 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_4CO1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CO1-query.scw PDB file : Tito_Scwrl_4CO1.pdb: