Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNMALKVSIASKSAHLTPEQIEEFGRRVEQIRQDVMQSLGEQDAKYIYKVRNFVRYTEIASRGMLMFGGWIPPVWLLGTGLLGISKIVENMELGHNVMHGQFDWLNEPSLNGNTYDWDTIASGDDWRETHNYVHHTYTNIVGKDHDIGYGILRVSDQQKWEPRHLFNIPLALQLMFFFEWYVGVQNLHLEDALVYKTKSWKQVWEDAAKVRKKATRQVLKDYVFFPVISGPMFLPVFAGNVVANIIRNLWSSAVIFNGHFTEDAETFEPDNTDTETKAEWYLRQIRGSSNFSGTEWLHFMSGNLSHQIEHHLFPDMPANRYKEVAPKIKALCAEYGINYNEANFMRQFWSVWVRLAKCSLPNHTTAKVMQTLEKLKAKFKFA 2F60 Chain:K ((3-28)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------HIPGTLRFRLSPAARNILEKHSLDAS------------------------------------------

General information: TITO was launched using: RESULT: Template: 2F60.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 28 -4566 -163.07 -175.62 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain K : 0.50 3D Compatibility (PKB) : -163.07 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.781

(partial model without unconserved sides chains): PDB file : Tito_2F60.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F60-query.scw PDB file : Tito_Scwrl_2F60.pdb: