Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKLNKLMLGLGLTVASVAANAAGYGVIDLAKVVESSTYLKQQNASLNQSVKPTTTKLEQLGKELERLQRQAQTQGQKMKEDEIKKLQSQYQSKLNEFNSTQQGLQSRVQTSLQSMNTTFETRVKQAAEQLRKENNLDFILNKNSTVAYDAKYDLTDKMIQKVNSMK 4KQT Chain:A ((22-169)) ------------------GPALPGVCIFSSPRAVGSSLVGKAVDARLKTIIQQVNAELTGERTALDNEAKALDAKKTTIAQDALEQQAATLQAKANAWQRKGQLRQKEVEATEQKALSRVYQELNTPIQQVYQAQKCSVLLDREAVMLANPAMDITDAVVAALDAR-

General information: TITO was launched using: RESULT: Template: 4KQT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 361 -41462 -114.85 -280.15 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -114.85 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.708

(partial model without unconserved sides chains): PDB file : Tito_4KQT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KQT-query.scw PDB file : Tito_Scwrl_4KQT.pdb: