Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTRTVQTLKNSTDLVQRFALPEIWEDFELRRLSNKDRYKHYILIFKNVVSQKKDWEDVKVVAEIQERNHNLRFNIKISKQYPELAAYEKLLEAKINAIINQSSLVIS 2Z61 Chain:A ((239-281)) -----------------------------------------------------------EIIEAILKLQQNLFISAPTISQYAALKAFEKETEREINSMIKE------

General information: TITO was launched using: RESULT: Template: 2Z61.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 20 2200 109.98 51.15 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 109.98 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.678

(partial model without unconserved sides chains): PDB file : Tito_2Z61.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z61-query.scw PDB file : Tito_Scwrl_2Z61.pdb: