Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIQIKQRPLLTRPEQFQGVPPFIAEILARRGVQSEQELELKLKNLLAPELKGLDAAVALMDQAIDEQKKIVIVGDYDADGATSTALMMLVLRDMGAQVDYLVPDRFKYGYGLTPAIAELAHHTYQPDLLITVDNGISSHAGVDAAHALGMQVIITDHHLTTKETPLAEAVVNPNQLGCEFPSK-ALAGVGVAFYVLANLASLRNRQGKSTSKVTQYLDLVALGTYADVAVLDYNNRILVDAGVKRIQQHQCRVGILALLDIAGREATSIRAQDLGFVLGPRINAAGRMESMRIGIECLLADTMETAYPIAQQLNQLNIDRRQIEGEMKQQALSALDSLQLSQQDIPAALVLFEENWHQGVIGIVAGRLKEQFHRPTIVFAPDEDGIHIKGSARSIDGVHIRDTIEQVAEQHPELVSHFGGHAAAAGLTIRKENFEAFKTVFNDCVAAMDESIFQATLWTDGELPVSALQLDTLNWIEQLGPWGQKFPLPQFEGYFKVIDFRWLKETHLKLRLAIDQYSFDAIAFNAAGRFEFDPMRDHVHLVYEIDRNVFNGNVSLQLRIVHLNQ 5F56 Chain:A ((18-475)) ----------LLATMREWQVSPP-VAQVLCGRDLRTEL-LALPLELTPNPALR--EAARHIV-AAVREGKRIRIHGDYDADGVSATATLVLGLRAIGANVHGFIPHRLNEGYGIHP--DRVPEHAAAADLVVTVDCGVSNLDEVKSLLATGTEVVVTDHHAPGENFPEC-LVVHPHLTPDYDPDRHNLTGAGVAYHL---LWAVYEELGRPEPRA--LLPLATLGTVADVAPLLGENRALVRAGLAEMARTEL-PGLRALMN--EKRVRQPTARDVAFILAPRINAAGRMGEADRALELLTTPSDHEAKSLAAYLEIRNQERRKIQDDMFAQA------LQLADPNDP-ALVLTHDDWHAGVMGIVASKLVETFNRPVYIVA------QGKGSVRSTPGI---SAVQGLRESR-DLLGRFGGHPGAAGFSLDPQNFGALRERIHGYVRQFPTPVPAVRL--DAPLPVAALTPELLSELSILEPFGEGNPRPLWHLRGPLTDTR----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5F56.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2639 -140585 -53.27 -307.62 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -53.27 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.504

(partial model without unconserved sides chains): PDB file : Tito_5F56.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F56-query.scw PDB file : Tito_Scwrl_5F56.pdb: