Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQFTEEQLLIRDMAKSFAQEQIKPNASDWDRDGTFPKETLTQMGQLGFMGMLVSEEWGGSDTGNLAYVLALEEVAAADGATSTIMSVHNSVGCVPILKFGTDEQKERFLKPLAQGEMIGAFALTEPHTGSDAAAIKTRAVKDGDDYILNGAKQFITSGNNAGVIIVFAVTDPSAGKKGISAFLVPRETPGYEVIRVEEKLGLHASDTCQIALTDVRIHKSLMLGKEGEGLKIALANLEGGRIGIAAQAVGLARAALEEATRYAKERITFGKPIFEHQTIAFRLASMATEIEAARQLVHYAARFKEVGQPCLNEASMAKLFASEMTERVCSSALQVFGGYGYLRDFPIERIYRDARICQIYEGTSDIQRLVIARSL 4N5F Chain:A ((13-385)) --YTEDQRMIRDAARAFATEMLAPNAAQWDHDAHLPDAIVAQLGELGLLGMIVPQELGGSYTDYVAYALAMEEVAAGDAACATMMSVHNSVGCGPILGFGTPAQKDRWLADMAAGRVIGAFCLTEPHAGSEANNLRTRAELRDGQWVLNGAKQFVTNGQRAGVAIVFAMTDPEAGKRGISAFLVPTDTPGFIVGKPEKKMGIRASDTCPITFENCAIPEDNLLGNRGEGLKIALSNLEGGRIGIAAQALGIARAAFDKARRYAGERVQFGKPIAEHQAIQQKLADMAVQINAARLLVHHAAKLRTAGLPCLSEASQAKLFASEMAERVCSDAIQIHGGYGYLVDYEVERHYRDARITQIYEGTSEVQRMVIARQL

General information: TITO was launched using: RESULT: Template: 4N5F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2238 -202733 -90.59 -543.52 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.90 3D Compatibility (PKB) : -90.59 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.529

(partial model without unconserved sides chains): PDB file : Tito_4N5F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4N5F-query.scw PDB file : Tito_Scwrl_4N5F.pdb: