Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQTIHGNRSHPKKSSHRLAGISSMVGTTIEWYDFFVYGAAAALIFNKLFFPNLDPLTGVLAAFATYAVGFIGRPLGGLVFGHFGDKIGRKSMLLLTLMLMGIPTVLIGLL-----------PTYESIGYWAAIGLVILRFIQGMAMGGEWGGAVLMAVEHAPEGGKGFWGSLPQASTGGGLMLASIALGLV-SLLPEQALFSWGWRLPFLASIILLAVGWYIRVKVPESPDF--------------------EKVKQQSEEVKVPAFQVLKNHPKQLIT----IILARAAENAWFYIASTFTLAYTTTQLGIP---RQDILFATICGAAVILFMTPLCGHLSDKVGQRNMFMFGLCVLALYSYPFFSMLNTKDPVLVWTAIVLAIGVVFPIMYAPQAQLFARQ-FPAEIRYSGISISVQ---LAGVLGGGLAPMI---ATKLLSIGQGNPYLIMYYIGSMAVVAIISTSFMPRDWSYEKDETYYKKANLNLKTKID 4GC0 Chain:A ((5-479)) ---------YNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVAPQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGSAWPELGFTSINPDNTVPVYLAG-YVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIRGKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLYTVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGCMGVLAALFMWKFVPETKGKTLEELEALWE-----------

General information: TITO was launched using: RESULT: Template: 4GC0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2217 -265006 -119.53 -617.73 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -119.53 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.365

(partial model without unconserved sides chains): PDB file : Tito_4GC0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GC0-query.scw PDB file : Tito_Scwrl_4GC0.pdb: