Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDQSTTSKARPMTSTQGSWFAILAVAIVAFALVTSEFLPVGVLNSVAADL----HISVGTAGLIITVPGIMAAIAAPLLPVSVKQLDRRYVLILLTAIMVIANTITAFAEN-------FHVLLLSRLILGISIGGFWATAIALSGKLAPANLPIAKATAVVMAGVTFATVLGVPIGTWLSEFYG--------------WRSAFGITAAIGLVVLVLQLIFLPKLLPESAIHIR--DLPALLRT----PKARSGMLIVLLIGLAHFCAYSYLAPFFKNVAGFNG----------TTISSLLLLYGIAGIFGNAFAGYSGNLNVRYTLAFV-GTCFAIVFFGFPIF---AIHEFGAIVLTALWGFAFGAFPTSANIWMFVHAPH-AVEKGMPLFVGMFQVMIATGSLLGGYIVDHFNENTLIYGVLSFVALALISTFTLAKGLNNPKATCEN 4J05 Chain:B ((33-498)) -----------------QIKLVLLAGVGFFLDAYDLFIINQVAPMLAQVYFPKTGLPAQRQDLMKAAANIGCVVGQVMFGVLGDSFGRKFVYGKELILIIVATIFQMSAPSHWDGNRVLTWITICRVFLGIGIGGDYPMSATVVSDRANIHR-RGTLLCFIFANQGWGSFVGSLVTIVTISGFKHRLKSGHTHDVDKAWRILIGLSLIPAFGTLYQ-R---------------WQEFVAYFSTWNHFRNLLGSMLGWFLVDIAFYGINLNQSVVLAQI-GFAGKTGDVYDKLFQLATGNIIVTALGFLPGYYFTLFLIDIVGRKKLQFMGFIMSGLFLAILAGEIDHIGKGPLLACFTFMQFFFNFGANTTTFIVAAELFPTRIRASAHGISAAAGKCGAILSSLVFNQLKAKIGTSAVLWIFFSTCILGFIST----------------

General information: TITO was launched using: RESULT: Template: 4J05.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1648 -214396 -130.09 -607.35 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -130.09 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.310

(partial model without unconserved sides chains): PDB file : Tito_4J05.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4J05-query.scw PDB file : Tito_Scwrl_4J05.pdb: