Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTNLAHKQEEDNVITLHPSTAKKKERTAMSDKFDKGYVMSSRLYRNEVKPFLGDAARNVYAELEEYISGFNKESDFVSYSQLQGKKIEGLEEHVRKLSTATVRAGLKQLIEYGVISIVATNPKLGNKYKLNEISLVEHFSNKSTSETKALQKLNSTTLETKAQGTLETKDTIDIIYRYLIIDNLFNSLRSNKPLENHFFVYQENKKQVFLEQQKLEAEEKAKAEKERKDKVRKLSFDEVIKLTKTTFANLCDLELWEQYVAFRSQKANTKLTKNALNVIYKDLKAWGFQGSNQSLKTAISGNYLGLFAPKQQNHGFVNQSQASTRMSEIQELIAKEEAGNEQYGF 1KU9 Chain:B ((1022-1076)) --------------------------------------------------HGLNKSVGAVYAILYLS--DKP-----LTISDIMEE---------LKISKGNVSMSLKKLEELGFVRKVWI---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1KU9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 180 -32378 -179.88 -588.69 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -179.88 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.680

(partial model without unconserved sides chains): PDB file : Tito_1KU9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KU9-query.scw PDB file : Tito_Scwrl_1KU9.pdb: